Transgene Course
Gain expertise in transgene design for tomato fungal disease resistance. The course covers construct architecture, transformation protocols, molecular validation, phenotyping assays, biosafety measures, and regulatory compliance to develop durable, safe resistant tomato varieties for biological research and applications.

flexible workload of 4 to 360h
valid certificate in your country
What will I learn?
This course offers a practical guide to designing, transforming, and validating tomato lines with strong resistance to fungal diseases. You will master construct design, promoter selection, RNAi and HIGS methods, transformation processes, regeneration techniques, molecular screening, phenotyping, and essential biosafety, risk assessment, and regulatory documentation for compliant outcomes.
Elevify advantages
Develop skills
- Design optimised transgene constructs with suitable promoters, markers, and targeting.
- Perform tomato transformation using Agrobacterium and efficient regeneration methods.
- Validate transgene expression via PCR, qPCR, RT-qPCR, Western blot, ELISA, and reporters.
- Conduct disease resistance assays with proper inoculation, phenotyping, and data analysis.
- Ensure biosafety compliance through risk assessment and regulatory documentation.
Suggested summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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