Restriction Enzymes Course
Gain confidence in using restriction enzymes for cloning. The course teaches primer design, vector selection, digest planning, ligation optimisation, transformation, and colony screening to build and confirm expression constructs reliably for your biology projects. It covers troubleshooting common problems and alternative methods for reproducible lab outcomes.

flexible workload of 4 to 360h
valid certificate in your country
What will I learn?
This course offers a quick and practical guide from designing primers to creating verified constructs using restriction enzymes. You will learn to prepare primers for restriction digestion, select suitable expression vectors, execute accurate digests, and improve ligation efficiency. Gain expertise in colony PCR, minipreps, diagnostic digests, troubleshooting issues, and alternative cloning techniques for consistent results in your lab work.
Elevify advantages
Develop skills
- Design restriction cloning with precise enzyme sites for directional inserts.
- Master PCR primers that are Tm-balanced, frame-safe, and include restriction sites.
- Select optimal vectors, tags, and promoters for efficient protein expression.
- Perform efficient digests, ligations, and E. coli transformations.
- Verify clones using diagnostic digests, colony PCR, and sequencing.
Suggested summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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