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Restriction Enzymes Course

Restriction Enzymes Course
flexible workload of 4 to 360h
valid certificate in your country

What will I learn?

This course offers a quick and practical guide from designing primers to creating verified constructs using restriction enzymes. You will learn to prepare primers for restriction digestion, select suitable expression vectors, execute accurate digests, and improve ligation efficiency. Gain expertise in colony PCR, minipreps, diagnostic digests, troubleshooting issues, and alternative cloning techniques for consistent results in your lab work.

Elevify advantages

Develop skills

  • Design restriction cloning with precise enzyme sites for directional inserts.
  • Master PCR primers that are Tm-balanced, frame-safe, and include restriction sites.
  • Select optimal vectors, tags, and promoters for efficient protein expression.
  • Perform efficient digests, ligations, and E. coli transformations.
  • Verify clones using diagnostic digests, colony PCR, and sequencing.

Suggested summary

Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.
Workload: between 4 and 360 hours

What our students say

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EmersonPolice Investigator
The course was essential to meet the expectations of my boss and the company I work for.
SilviaNurse
Very great course. Lots of rich information.
WiltonCivil Firefighter

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