Electrophoresis Course
Gain mastery in electrophoresis techniques starting from basic principles up to producing publication-quality data. The course covers agarose gels, SDS-PAGE, western blotting, PCR and RFLP design, troubleshooting common problems, and proper documentation to ensure reliable and quantitative results suitable for any molecular biology lab setting.

flexible workload of 4 to 360h
valid certificate in your country
What will I learn?
This course provides hands-on skills to prepare and perform agarose gel electrophoresis, SDS-PAGE, and western blotting effectively. You will understand fundamental principles, select appropriate gels and buffers, design PCR products, apply RFLP methods, optimise procedures, troubleshoot issues, analyse quantitative protein data, and document results for reproducible and publishable outcomes in molecular biology.
Elevify advantages
Develop skills
- Plan agarose and SDS-PAGE experiments by selecting gel percentages, buffers, ladders, and voltages efficiently.
- Design PCR and RFLP assays choosing suitable primers, amplicon sizes, and markers for distinct bands.
- Perform superior western blots by optimising transfer, blocking, antibody use, and detection steps.
- Quickly troubleshoot gel and blot issues like smearing, faint bands, or high background.
- Document and store experiments properly by naming files, preserving gels and blots, and recording metadata.
Suggested summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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