RNA Sequencing Course
Gain mastery in RNA sequencing for biomedical applications: design reliable drug-response experiments, ensure top-quality data processing, align and quantify transcripts, perform differential expression analysis using DESeq2 or edgeR, and interpret pathways to support translational research and precision medicine initiatives.

flexible workload of 4 to 360h
valid certificate in your country
What will I learn?
This course delivers a complete, hands-on workflow for high-quality RNA-Seq in drug research. Master experimental design, quality checks and trimming, alignment or pseudoalignment, gene counting, and normalisation using DESeq2, edgeR, or limma-voom. Additionally, explore differential expression, pathway analysis, enrichment studies, visualisation techniques, and reporting for informed decisions.
Elevify advantages
Develop skills
- Design robust RNA-Seq drug studies with proper controls, replicates, and batch effects management.
- Perform efficient RNA-Seq QC, trimming, alignment, and accurate gene counting.
- Execute DESeq2 workflows for normalisation, FDR control, and identifying drug response DEGs.
- Analyse pathway and gene set enrichment to uncover drug mechanisms in cancer.
- Create compelling RNA-Seq visualisations and slides for impactful biomedical presentations.
Suggested summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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