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Restriction Enzymes Course

Restriction Enzymes Course
from 4 to 360h flexible workload
valid certificate in your country

What will I learn?

Gain a quick practical guide from primer design to confirmed constructs using restriction enzymes. Master designing primers for restriction sites, selecting expression vectors, executing accurate digests, and perfecting ligations. Excel in colony PCR, minipreps, diagnostic digests, troubleshooting issues, and alternative cloning techniques for dependable results in your lab work.

Elevify advantages

Develop skills

  • Design restriction cloning strategies with exact enzyme sites for directional inserts.
  • Master PCR primers that balance melting temperatures, maintain reading frames, and include restriction sites.
  • Select optimal vectors, fusion tags, and promoters for efficient protein expression.
  • Perform effective restriction digests, ligations, and bacterial transformations.
  • Verify cloned constructs using diagnostic digests, colony PCR, and DNA sequencing.

Suggested summary

Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.
Workload: between 4 and 360 hours

What our students say

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Very great course. Lots of rich information.
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