DNA Structure Course
Gain mastery over DNA structure, starting from the double helix and base pairing, through calculating Tm and GC content, to reverse complements. Understand how replication fidelity, mismatches, and primer design affect PCR, cloning, and sequencing processes—perfect for progressing in molecular biology and related fields.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
This course provides a quick, hands-on understanding of DNA double helix basics, base pairing rules, and thermodynamics, applying them to practical lab tasks. You will learn to analyse sequences, compute GC content and melting temperature, create reverse complements, spot structural issues, and grasp replication accuracy. By the end, you can confidently design primers, fix PCR problems, enhance sequencing results, and prepare for cloning experiments.
Elevify advantages
Develop skills
- DNA sequence analysis: rapidly calculate GC%, Tm, and identify problematic motifs.
- Complement design: produce precise complementary and reverse-complement sequences efficiently.
- PCR optimisation: create reliable primers, preventing dimers and hairpins.
- Replication fidelity: analyse mismatches, proofreading, and MMR effects.
- Lab cloning preparation: use base pairing for sticky, blunt, and adapter ligations.
Suggested summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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