Electrophoresis Course
Gain expertise in electrophoresis techniques starting from basic principles through to producing reliable, quantitative data suitable for publication. The course covers agarose gels, SDS-PAGE, western blotting, PCR and RFLP design, troubleshooting, and thorough documentation for molecular biology lab work.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
This course equips you with hands-on skills to set up and perform agarose gels, SDS-PAGE, and western blots effectively. You will grasp fundamental electrophoresis principles, select appropriate gels and buffers, design PCR products, apply RFLP methods, optimise procedures, troubleshoot issues, analyse quantitative protein data, and document findings for reproducible, publication-quality DNA and protein outcomes.
Elevify advantages
Develop skills
- Plan agarose and SDS-PAGE experiments by selecting gel percentages, buffers, ladders, and voltages efficiently.
- Design PCR and RFLP assays choosing suitable primers, amplicon sizes, and markers for distinct bands.
- Perform superior western blots optimising transfer, blocking, antibodies, and detection steps.
- Quickly troubleshoot gel and blot problems like smearing, faint bands, or excessive background.
- Document and store experiments properly by naming files, preserving gels and blots, and recording metadata.
Suggested summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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