RNA Sequencing Course
Gain mastery in RNA sequencing for biomedical applications: design reliable drug-response experiments, ensure quality reads, align and quantify transcripts, perform differential expression analysis using DESeq2 or edgeR, and interpret pathways to support translational and precision medicine research.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
This course delivers a complete workflow for high-quality RNA-Seq in drug studies, covering experimental design, quality control and trimming, alignment or pseudoalignment, gene counting, normalization with DESeq2, edgeR, or limma-voom, plus differential expression, pathway analysis, enrichment, visualization, and reporting for informed decisions.
Elevify advantages
Develop skills
- Design robust RNA-Seq drug studies with proper controls, replicates, and batch handling.
- Perform efficient RNA-Seq QC, trimming, alignment, and gene counting.
- Execute DESeq2 workflows for normalization, FDR control, and identifying drug response genes.
- Analyze pathways and gene set enrichment to uncover drug mechanisms in cancer.
- Create compelling RNA-Seq visualizations and slides for impactful biomedical presentations.
Suggested summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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