Restriction Enzymes Course
Master restriction enzymes for confident cloning. Design primers, choose vectors, plan digests, and troubleshoot ligation, transformation, and colony screening to reliably build and verify expression constructs in your biological sciences projects.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
The Restriction Enzymes Course gives you a fast, practical path from primer design to verified constructs. Learn to design restriction-ready primers, choose expression vectors, plan precise digests, and optimize ligations. Master colony PCR, minipreps, and diagnostic digests, then troubleshoot failures and explore alternative cloning methods for reliable, reproducible results in your own lab projects.
Elevify advantages
Develop skills
- Restriction cloning design: plan precise enzyme sites for clean, directional inserts.
- PCR primer mastery: build Tm-balanced, frame-safe primers with restriction sites.
- Vector strategy: choose optimal plasmids, tags, and promoters for fast expression.
- Ligation and transformation: set up efficient digests, ligations, and E. coli uptake.
- Clone verification: use digests, colony PCR, and sequencing to confirm constructs.
Suggested summary
Before starting, you can change the chapters and workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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