Transgene Course
Gain expertise in creating transgene-based tomato lines resistant to fungal diseases. The course covers construct design, Agrobacterium transformation, regeneration techniques, molecular validation like PCR and qPCR, disease phenotyping assays, and essential biosafety plus regulatory compliance to develop safe, effective resistant varieties for biological research and applications.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
This course provides a practical guide to designing, transforming, and validating tomato lines with lasting resistance to fungal diseases. You'll master construct design, promoter selection, RNAi and HIGS methods, transformation processes, molecular checks, phenotyping, plus biosafety, risk evaluation, and regulatory paperwork for compliant outcomes.
Elevify advantages
Develop skills
- Design effective transgene constructs with optimal promoters, markers, and targeting.
- Perform tomato transformation using Agrobacterium and efficient regeneration methods.
- Validate transgenes via PCR, qPCR, RT-qPCR, Western blot, ELISA, and reporters.
- Conduct disease resistance assays with proper inoculation, phenotyping, and data analysis.
- Ensure biosafety compliance through risk assessments and regulatory documentation.
Suggested summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
Your lessons are perfect. I purchased the one-year package and finally have the opportunity to follow various topics of my interest without needing to change platforms... I thank you for everything you do, I've already recommended you to other people...

Giulio CarloDigital Marketing Student
I like how the lessons are straight to the point and how I can change chapters and skip content I don't need.

Mariana FerresPhotography Student
I like the content and the way videos are presented and transcribed, which speeds up the process!

Luciana AlvarengaNail Design Student
The platform is fast, simple to use. The diversity of content and complementary videos help a lot with learning.

André FelipePrompt Engineering Student
FAQ
Who is Elevify? How does it work?
Do the courses have certificates?
Are the courses free?
What is the course workload?
What are the courses like?
How do the courses work?
What is the duration of the courses?
What is the cost or price of the courses?
What is an EAD or online course and how does it work?
PDF Course