Transgene Course
Gain expertise in creating transgene-based tomato lines resistant to fungal diseases. The course covers construct design, Agrobacterium transformation, regeneration techniques, molecular validation like PCR and qPCR, disease phenotyping assays, and essential biosafety plus regulatory compliance to develop safe, effective resistant varieties for biological research and applications.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
This course provides a practical guide to designing, transforming, and validating tomato lines with lasting resistance to fungal diseases. You'll master construct design, promoter selection, RNAi and HIGS methods, transformation processes, molecular checks, phenotyping, plus biosafety, risk evaluation, and regulatory paperwork for compliant outcomes.
Elevify advantages
Develop skills
- Design effective transgene constructs with optimal promoters, markers, and targeting.
- Perform tomato transformation using Agrobacterium and efficient regeneration methods.
- Validate transgenes via PCR, qPCR, RT-qPCR, Western blot, ELISA, and reporters.
- Conduct disease resistance assays with proper inoculation, phenotyping, and data analysis.
- Ensure biosafety compliance through risk assessments and regulatory documentation.
Suggested summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
FAQs
Who is Elevify? How does it work?
Do the courses have certificates?
Are the courses free?
What is the course workload?
What are the courses like?
How do the courses work?
What is the duration of the courses?
What is the cost or price of the courses?
What is an EAD or online course and how does it work?
PDF Course