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Restriction Enzymes Course

Restriction Enzymes Course
from 4 to 360h flexible workload
valid certificate in your country

What will I learn?

This course offers a quick, hands-on guide from designing primers to confirming constructs using restriction enzymes. You'll learn to create primers ready for restriction, select suitable expression vectors, execute accurate digests, and perfect ligations. Gain expertise in colony PCR, minipreps, diagnostic digests, troubleshooting issues, and alternative cloning techniques for dependable results in your lab work.

Elevify advantages

Develop skills

  • Design restriction cloning strategies with exact enzyme sites for directional inserts.
  • Master PCR primers that balance melting temperatures, maintain reading frames, and include restriction sites.
  • Select ideal plasmids, fusion tags, and promoters for efficient protein expression.
  • Perform effective restriction digests, ligations, and transformations into E. coli.
  • Verify clones accurately using diagnostic digests, colony PCR, and DNA sequencing.

Suggested summary

Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.
Workload: between 4 and 360 hours

What our students say

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Very great course. Lots of rich information.
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