Transgene Course
Gain expertise in creating transgene-enabled tomato varieties resistant to fungal diseases. The course covers transgene construct design, Agrobacterium-mediated transformation, molecular confirmation techniques like PCR and qPCR, disease phenotyping assays, biosafety protocols, and regulatory documentation to develop compliant, high-performing resistant lines for biological research and applications in Zambia's agricultural context.

from 4 to 360h flexible workload
certificate valid in your country
What will I learn?
This course provides a practical guide to designing, transforming, and validating tomato lines with lasting resistance to fungal diseases. Key topics include transgene construct design, promoter selection, RNAi and HIGS methods, transformation protocols, regeneration techniques, molecular validation, phenotyping, and essential biosafety, risk assessment, plus regulatory compliance for successful outcomes.
Elevify advantages
Develop skills
- Design effective transgene constructs with optimal promoters, markers, and targeting signals.
- Perform tomato transformation using Agrobacterium methods and efficient regeneration processes.
- Validate transgene activity through PCR, qPCR, RT-qPCR, Western blot, ELISA, and reporter assays.
- Conduct disease resistance tests with proper inoculation, phenotyping, and statistical analysis.
- Implement biosafety measures, risk evaluations, and regulatory dossier preparation for compliance.
Suggested summary
Before starting, you can change the chapters and workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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