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Restriction Enzymes Course

Restriction Enzymes Course
from 4 to 360h flexible workload
certificate valid in your country

What will I learn?

This course offers a quick practical guide from primer design to verified constructs using restriction enzymes. You will design primers ready for restriction, select expression vectors, plan accurate digests, optimise ligations, master colony PCR, minipreps and diagnostic digests, troubleshoot issues, and learn alternative cloning techniques for dependable reproducible results in your laboratory work.

Elevify advantages

Develop skills

  • Design restriction cloning with precise enzyme sites for clean directional inserts.
  • Master PCR primers that are Tm-balanced, frame-safe with restriction sites.
  • Select optimal plasmids, tags and promoters for rapid expression.
  • Perform efficient digests, ligations and E. coli transformations.
  • Verify clones using digests, colony PCR and sequencing.

Suggested summary

Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.
Workload: between 4 and 360 hours

What our students say

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EmersonPolice Investigator
The course was essential to meet the expectations of my boss and the company I work for.
SilviaNurse
Great course. A lot of valuable information.
WiltonCivil Firefighter

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