Transgene Course
Gain expertise in creating transgene constructs for tomato fungal resistance. The course covers construct building, plant transformation techniques, molecular validation, disease testing protocols, biosafety measures, and regulatory requirements to develop reliable, disease-resistant tomato varieties suitable for biological research and applications in Uganda's agricultural context.

flexible workload from 4 to 360h
valid certificate in your country
What will I learn?
This course offers a practical guide to designing, transforming, and validating tomato lines with lasting resistance to fungal diseases. You will master construct design, promoter selection, RNAi and HIGS methods, transformation processes, molecular checks, phenotyping, plus biosafety, risk evaluation, and regulatory paperwork for compliant outcomes.
Elevify advantages
Develop skills
- Design effective transgene constructs with optimal promoters, markers, and targeting.
- Perform tomato transformation using Agrobacterium and efficient regeneration methods.
- Confirm transgene activity through PCR, qPCR, RT-qPCR, Western blot, ELISA, and reporters.
- Conduct disease resistance tests with proper inoculation, phenotyping, and data evaluation.
- Implement biosafety protocols, risk assessments, and regulatory documentation preparation.
Suggested summary
Before starting, you can change the chapters and workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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