Restriction Enzymes Course
Gain confidence with restriction enzymes for effective cloning. Design primers, pick vectors, set up digests, and handle problems in ligation, transformation, and colony screening to build and check expression constructs reliably in your biology projects. The course builds practical skills for real lab success, ensuring you achieve precise results every time with minimal trial and error.

flexible workload from 4 to 360h
valid certificate in your country
What will I learn?
This course offers a quick, hands-on guide from primer design to confirmed constructs. You will learn to create primers ready for restriction, select expression vectors, arrange accurate digests, and improve ligations. Gain expertise in colony PCR, minipreps, and diagnostic digests, plus how to fix issues and try other cloning techniques for steady, repeatable outcomes in your lab work.
Elevify advantages
Develop skills
- Design restriction cloning: plan exact enzyme sites for neat, directional inserts.
- Master PCR primers: create balanced Tm, frame-safe primers with restriction sites.
- Strategise vectors: select best plasmids, tags, and promoters for quick expression.
- Handle ligation and transformation: prepare effective digests, ligations, and E. coli uptake.
- Verify clones: apply digests, colony PCR, and sequencing to validate constructs.
Suggested summary
Before starting, you can change the chapters and workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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