Transgene Course
Gain expertise in creating transgene-based tomato lines resistant to fungal diseases. The course covers transgene construct design, Agrobacterium-mediated transformation, regeneration protocols, molecular confirmation via PCR and protein assays, disease challenge testing, data analysis, and full biosafety plus regulatory documentation to ensure safe, effective, and compliant resistant varieties for biological research and application.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
This course provides a practical guide to designing, transforming, and validating tomato plants with strong resistance to fungal diseases. You will master transgene construct design, promoter selection, RNAi and HIGS methods, plant transformation techniques, molecular validation, disease phenotyping, and essential biosafety, risk evaluation, and regulatory compliance for successful outcomes.
Elevify advantages
Develop skills
- Design effective transgene constructs with optimal promoters, selection markers, and targeted protein expression.
- Perform tomato transformation using Agrobacterium methods and efficient plant tissue regeneration techniques.
- Validate transgene integration and expression through PCR, qPCR, RT-qPCR, Western blot, ELISA, and reporter assays.
- Conduct disease resistance assays with proper inoculation, phenotyping protocols, and statistical data analysis.
- Implement biosafety measures, conduct risk assessments, and prepare comprehensive regulatory dossiers.
Suggested summary
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