DNA Structure Course
Gain mastery over DNA structure, starting from the double helix and base pairing, through calculating Tm and GC content, to reverse complements. Understand how replication fidelity, mismatches, and primer design affect PCR, cloning, and sequencing processes. Perfect for boosting skills in molecular biology and related fields, equipping you for real lab challenges.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
This course provides a quick and practical understanding of DNA double helix basics, base pairing rules, and thermodynamics. You will learn to analyze sequences, compute GC content and melting temperature, create reverse complements, spot structural issues, and grasp replication accuracy. By the end, you can confidently design primers, fix PCR problems, enhance sequencing results, and prepare for cloning tasks.
Elevify advantages
Develop skills
- DNA sequence analysis: rapidly calculate GC content, Tm, and identify problematic motifs.
- Complement strand design: produce precise complementary and reverse-complement sequences efficiently.
- PCR primer optimization: create reliable primers while preventing dimers and hairpins.
- Replication accuracy: analyze mismatches and effects of proofreading and mismatch repair.
- Cloning techniques: use base pairing for sticky ends, blunt ends, and adapter ligations in the lab.
Suggested summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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