Lesson 1Selecting in vitro models: organoids, co-culture systems, 2D vs 3D scaffolds and disease-relevant readoutsWe talk picking and making lab models, like flat grows, 3D frames, mini-organs, and joint grows, stressing surround hints, sickness-linked checks, speed, and match with body findings.
Flat layer vs 3D grow setupsMini-organ models for organ sicknessesJoint grow and guard-stroma talksSupport frame and frame pushTest checks linked to body resultsSpeed, grow big, test strongLesson 2Small animal models: mouse and rat disease models relevant to chosen indication and translatability limitsWe focus on small beast models, mostly mouse and rat, describing sickness models fit for mend needs, gene tools, guard backs, easy points, and main limits for guessing human safety and good work.
Mouse vs rat: strong and weakNo-guard and human-like small beast modelsCommon sickness models for key needsModel true and fit to humansLimits in size, guard, life lengthLink data to big beasts and clinicsLesson 3Dosing strategies and kinetics: cell dose-ranging studies, biodistribution, and persistence analysisWe explain amount plans for cell heals, including amount-range makes, one vs repeat amounts, path-based tweaks, spread mapping, lasting and stick check, and model show-answer links.
Make of amount-up and range-findOne vs many amount plansMark cells for body trackSpread check and organ pullLasting, stick, clear movesShow-answer and safety edge modelLesson 4Large animal models: pig, sheep, non-human primate choices for scale-up and functional similarityWe look at big beast models like pig, sheep, and ape-like, focusing body and work match, guard setup, home and care, size of tools and amounts, and when big models key for move.
Rules for picking big beast kindsPig models for heart and liver mendSheep models for bone and back studiesApe-like models and right waysSize of tools, amounts, stepsCare, well, rule watchLesson 5Outcome measures: histology, functional assays, imaging modalities (MRI, PET, bioluminescence), molecular biomarkers, and behavioral testsWe detail picking and true result checks, like tissue looks, work tests, picture ways, tiny body signs, and act tests, stressing feel, true spot, count, and fit to human clinic good.
Tissue score and shape checkLab and out work testsMRI, PET, light picture plansTiny body signs and all-checksAct tests and work showTrue, same, test cleanLesson 6Delivery routes and device-assisted delivery: intravenous, intra-organ, intra-articular, biomaterial scaffolds, and catheter-based techniquesWe review give paths and tool-help ways for cell and gene-tweak goods, like blood way, in-organ, in-joint, frame-based, and tube ways, highlighting aim good, safety, tech easy.
Blood and blood-way giveStraight in-organ and deep shotIn-joint and in-disc waysSupport frame and wet gelsTube-based and picture-guide giveStep-linked dangers and cut-backLesson 7Study design principles: hypotheses, controls, randomization, blinding, sample size calculation, and statistical endpointsWe bring in strict pre-clinic study make, from setting guesses and picking controls to random pick, hide, group size count, and number ends, to cut lean and better same and rule take.
Making test way guessesPick of control and compare groupsRandom plans and hide giveHide of finders and result checkersGroup size and power countsMain, side, find endsLesson 8Cell sourcing, manufacturing and GMP considerations for preclinical batches: isolation, expansion, cryopreservation, and release criteriaWe cover getting and making pre-clinic cell batches, like tissue pick, get out, grow, freeze hold, quality check, GMP follow, and let-out rules to sure same, safe, well-known goods.
Giver pick and tissue getCell get out and early markGrow, pass, tiredness controlFreeze hold ways and warm backGMP papers and batch notesLet-out test and rule set
