High-Performance Liquid Chromatography (HPLC) Course
Gain mastery in High-Performance Liquid Chromatography (HPLC) tailored for multi-component pharmaceuticals. The course covers essential techniques including column and mobile phase selection, gradient optimization, detection strategies, sample preparation, calibration, method validation for precision and accuracy, troubleshooting common problems, and producing robust, regulatory-compliant documentation suitable for demanding lab settings.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
This HPLC course builds practical skills for developing and refining methods for multi-component drugs. You will learn to select columns and mobile phases, design gradients, prepare samples, perform calibrations with internal standards, validate methods for precision, accuracy, LOD/LOQ, troubleshoot issues, ensure robustness, and prepare regulatory documentation in an efficient format.
Elevify advantages
Develop skills
- Develop HPLC methods to create fast, reliable assays for multi-component tablets.
- Master mobile phases by adjusting pH, buffers, and gradients for clear, sharp peaks.
- Optimize detectors such as UV, DAD, ELSD, or MS for accurate quantitation.
- Master validation to confirm accuracy, precision, LOD, and LOQ per regulatory guidelines.
- Acquire troubleshooting skills to quickly resolve tailing, drift, and pressure issues.
Suggested summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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