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Electrophoresis Course

Electrophoresis Course
from 4 to 360h flexible workload
valid certificate in your country

What will I learn?

This course equips you with hands-on skills to prepare and perform agarose gels, SDS-PAGE, and western blots effectively. You will grasp essential electrophoresis principles, select appropriate gels and buffers, design PCR products, apply RFLP methods, and master optimization, troubleshooting, protein quantification, data analysis, and record-keeping for reliable, reproducible DNA and protein outcomes suitable for publication.

Elevify advantages

Develop skills

  • Plan agarose and SDS-PAGE experiments by selecting gel percentages, buffers, ladders, and voltages efficiently.
  • Design PCR and RFLP assays choosing suitable primers, amplicon sizes, and markers for distinct band patterns.
  • Perform superior western blots by fine-tuning transfer conditions, blocking steps, antibody use, and detection methods.
  • Quickly troubleshoot issues in gels and blots such as smearing, faint bands, or excessive background.
  • Document and store experiments properly by organizing file names, preserving gels and blots, and recording metadata.

Suggested summary

Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.
Workload: between 4 and 360 hours

What our students say

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EmersonPolice Investigator
The course was essential to meet the expectations of my boss and the company where I work.
SilviaNurse
Very great course. Lots of rich information.
WiltonCivil Firefighter

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