Electrophoresis Course
Gain expertise in electrophoresis techniques starting from basic principles through to producing high-quality, publication-standard data. The course covers agarose gel electrophoresis, SDS-PAGE, western blotting, PCR and RFLP assay design, troubleshooting common issues, and proper documentation practices, enabling you to achieve consistent and quantitative results in molecular biology labs anywhere.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
This course equips you with hands-on skills to prepare and perform agarose gels, SDS-PAGE, and western blots effectively. You will grasp essential electrophoresis principles, select appropriate gels and buffers, design PCR products, apply RFLP methods, and master optimization, troubleshooting, protein quantification, data analysis, and record-keeping for reliable, reproducible DNA and protein outcomes suitable for publication.
Elevify advantages
Develop skills
- Plan agarose and SDS-PAGE experiments by selecting gel percentages, buffers, ladders, and voltages efficiently.
- Design PCR and RFLP assays choosing suitable primers, amplicon sizes, and markers for distinct band patterns.
- Perform superior western blots by fine-tuning transfer conditions, blocking steps, antibody use, and detection methods.
- Quickly troubleshoot issues in gels and blots such as smearing, faint bands, or excessive background.
- Document and store experiments properly by organizing file names, preserving gels and blots, and recording metadata.
Suggested summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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