Transgene Course
Gain expertise in transgene technology for tomato fungal resistance. The course covers construct architecture, Agrobacterium transformation, regeneration techniques, molecular validation via PCR and protein assays, disease phenotyping, and essential biosafety plus regulatory compliance to develop durable, safe resistant varieties for biological research and application.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
This course offers a practical guide to designing, transforming, and validating tomato lines resistant to fungal diseases. Learners master construct design, promoter selection, RNAi and HIGS methods, transformation processes, molecular screening, phenotyping, plus biosafety, risk evaluation, and regulatory documentation for compliant outcomes.
Elevify advantages
Develop skills
- Design optimised transgene constructs with suitable promoters, markers, and targeting.
- Perform tomato transformation using Agrobacterium and efficient regeneration methods.
- Validate transgenes through PCR, qPCR, RT-qPCR, Western blot, ELISA, and reporter assays.
- Conduct disease resistance tests with proper inoculation, phenotyping, and data evaluation.
- Ensure biosafety compliance via risk assessments and regulatory documentation preparation.
Suggested summary
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