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Restriction Enzymes Course

Restriction Enzymes Course
from 4 to 360h flexible workload
valid certificate in your country

What will I learn?

This course offers a quick, hands-on guide from primer design to confirmed constructs. You will learn to create primers ready for restriction, select expression vectors, arrange exact digests, and improve ligations. Gain expertise in colony PCR, minipreps, and diagnostic digests, plus how to fix issues and try other cloning techniques for steady, repeatable outcomes in your lab work.

Elevify advantages

Develop skills

  • Design restriction cloning: arrange exact enzyme sites for neat, directional inserts.
  • Master PCR primers: create balanced Tm, frame-safe primers with restriction sites.
  • Strategise vectors: select best plasmids, tags, and promoters for quick expression.
  • Handle ligation and transformation: prepare effective digests, ligations, and E. coli uptake.
  • Verify clones: apply digests, colony PCR, and sequencing to validate constructs.

Suggested summary

Before starting, you can change the chapters and workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload
Workload: between 4 and 360 hours

What our students say

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EmersonPolice Investigator
The course was essential to meet the expectations of my boss and the company where I work.
SilviaNurse
Very great course. Lots of rich information.
WiltonCivil Firefighter

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