CRISPR Course
This course offers a practical guide to designing and optimizing CRISPR-based single-base edits for treating monogenic blood disorders, covering guide RNA selection, editing strategies, ex vivo workflows, validation assays, and ethical considerations.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
This CRISPR Course provides a focused, practical guide to design and optimise precise single-base edits for monogenic blood disorders. You will learn guide RNA selection, repair template design, base and prime editing options, ex vivo HSC workflows, and delivery methods, plus strong molecular and functional assays, off-target and genotoxicity analysis, and key safety, ethical, and regulatory considerations.
Elevify advantages
Develop skills
- Design CRISPR guides: select PAMs, score on-targets, and limit bystander edits.
- Engineer precise single-base fixes: choose HDR, base editing, or prime editing.
- Optimise HSC ex vivo editing: RNP delivery, culture conditions, and scale-up.
- Validate edits rigorously: NGS, allele-specific assays, and functional readouts.
- Control off-targets and ethics: safety assays, risk review, and compliance.
Suggested summary
Before starting, you can change the chapters and workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workloadWhat our students say
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