Transgene Course
This course equips you with essential techniques to engineer tomato plants resistant to fungal diseases through transgene technology. Covering construct design, transformation methods, validation assays, disease phenotyping, and regulatory standards, it prepares you to develop compliant, durable resistant varieties for biological research and applications.

4 to 360 hours of flexible workload
certificate valid in your country
What Will I Learn?
Gain practical skills in designing transgene constructs, selecting promoters, using RNAi and HIGS for fungal resistance in tomatoes. Master Agrobacterium-mediated transformation, plant regeneration, molecular validation via PCR and phenotyping, plus biosafety protocols and regulatory compliance for successful tomato lines.
Elevify Advantages
Develop Skills
- Design optimised transgene constructs with suitable promoters, markers and targeting.
- Perform tomato transformation using Agrobacterium and efficient regeneration techniques.
- Validate transgenes with PCR, qPCR, Western blot, ELISA and reporter assays.
- Conduct disease resistance assays including inoculation, phenotyping and data evaluation.
- Implement biosafety measures, risk assessments and regulatory documentation.
Suggested Summary
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