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Restriction Enzymes Course

Restriction Enzymes Course
4 to 360 hours of flexible workload
certificate valid in your country

What Will I Learn?

This course offers a streamlined practical guide from primer design to verified constructs using restriction enzymes. You will learn to create primers ready for restriction, select suitable expression vectors, execute accurate digests, optimise ligations, perform colony PCR, minipreps and diagnostic checks, troubleshoot issues, and explore other cloning techniques for consistent results in your lab work.

Elevify Advantages

Develop Skills

  • Design restriction cloning with precise enzyme sites for clean directional inserts.
  • Master PCR primers that are Tm-balanced, frame-safe with restriction sites.
  • Select optimal vectors, tags and promoters for efficient expression.
  • Perform efficient digests, ligations and E. coli transformations.
  • Verify clones using digests, colony PCR and sequencing.

Suggested Summary

Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.
Workload: between 4 and 360 hours

What our students say

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EmersonPolice Investigator
The course was essential to meet the expectations of my boss and the company I work for.
SilviaNurse
Great course. Lots of valuable information.
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