Restriction Enzymes Course
Gain confidence in using restriction enzymes for cloning. Learn to design primers, select vectors, execute digests, troubleshoot ligations and transformations, and screen colonies to build and confirm expression constructs reliably in your biology projects. This course provides practical skills for lab success.

4 to 360 hours of flexible workload
certificate valid in your country
What Will I Learn?
This course offers a streamlined practical guide from primer design to verified constructs using restriction enzymes. You will learn to create primers ready for restriction, select suitable expression vectors, execute accurate digests, optimise ligations, perform colony PCR, minipreps and diagnostic checks, troubleshoot issues, and explore other cloning techniques for consistent results in your lab work.
Elevify Advantages
Develop Skills
- Design restriction cloning with precise enzyme sites for clean directional inserts.
- Master PCR primers that are Tm-balanced, frame-safe with restriction sites.
- Select optimal vectors, tags and promoters for efficient expression.
- Perform efficient digests, ligations and E. coli transformations.
- Verify clones using digests, colony PCR and sequencing.
Suggested Summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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