PCR Techniques Course
Gain comprehensive expertise in PCR and qPCR techniques, from assay design and nucleic acid extraction to contamination prevention, optimised thermal cycling, plate layouts, data analysis, and quality documentation. Produce reliable, audit-compliant results for high-throughput lab environments, covering the full workflow from sample intake to final reporting.

4 to 360 hours of flexible workload
certificate valid in your country
What Will I Learn?
Acquire hands-on skills in PCR and qPCR assay design, nucleic acid extraction methods, contamination prevention strategies, thermal cycling optimisation, protocol validation, plate layout planning, control usage and interpretation, troubleshooting of suboptimal results, and quality management practices to ensure defensible, high-quality data in laboratory settings.
Elevify Advantages
Develop Skills
- Design effective PCR/qPCR assays by selecting optimal targets, primers, and probes.
- Execute contamination-free nucleic acid extractions to preserve sample integrity.
- Optimise qPCR setups including master mixes, thermal cycling, and plate layouts.
- Analyse PCR/qPCR results with accurate Ct values, controls, and quality checks.
- Implement robust lab systems for documentation, SOPs, and biosafety compliance.
Suggested Summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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