Lesson 1Escalation plan for increasing aggression or panic: stopping procedure, safe withdrawal, sedation criteria, re-scheduling and documentationPrepare a graded response plan for rising fear, aggression, or panic. Learn criteria for pausing, aborting, or switching to sedation, safe withdrawal techniques, and how to document and reschedule procedures ethically.
Early warning signs of escalationCriteria for pausing or stoppingSafe disengagement and room exitWhen to recommend chemical sedationRescheduling and case flaggingDebriefing and protocol adjustmentLesson 2Stress-reduction measures during procedure: low-voice cues, counterconditioning, timed breaks, analgesia or anxiolytic considerations (protocol discussion only)Apply stress-reduction tools during handling, including calm voice, predictable touch, and treat-based counterconditioning. Plan brief breaks, consider analgesia or anxiolytics, and avoid common actions that heighten fear.
Low, steady voice and breathing controlTreat delivery and cooperative careGentle touch and pressure techniquesPlanned pauses and short breaksAnalgesia and anxiolytic indicationsBehaviors that increase canine stressLesson 3Bite and scratch risk mitigation: safe hand placement, protective equipment choices, muzzling technique and fit checksReduce bite and scratch risk through correct hand placement, body positioning, and use of protective gear. Learn when and how to apply basket or cloth muzzles, verify fit, and monitor breathing and stress while restrained.
Safe zones for hands and forearmsBody positioning to avoid the bite arcSelecting gloves and arm protectionChoosing basket versus cloth muzzleFitting and checking muzzle securityMonitoring breathing and distress signsLesson 4Recordkeeping, consent, and post-procedure monitoring: monitoring vitals, bandage application, dispelling dog, and incident reportingDocument consent, planned procedures, and behavior notes before handling. After the draw, monitor vitals, check the venipuncture site and bandage, record any incidents, and give owners clear discharge and follow-up instructions.
Obtaining informed owner consentBaseline vitals and pre-procedure notesBandage placement and site inspectionShort-term monitoring after venipunctureBehavior and incident documentationOwner communication and home careLesson 5Restraint options and selection criteria: lateral recumbency vs sitting restraint, use of slip or basket muzzle, head restraint, towel wrap methodsCompare restraint options for a nervous medium dog, including sitting, standing, and lateral recumbency. Learn when to add a muzzle or towel wrap, and how to balance safety, sample quality, and patient emotional welfare.
Indications for sitting restraintWhen to use lateral recumbencySlip lead versus basket muzzle useHead control without overpressureTowel wrap methods for added controlBalancing safety and welfare needsLesson 6Approach technique and initial contact: slow movement, side-on approach, hand signals, offering treats and towel introductionUse a slow, side-on approach to gain trust before restraint. Coordinate body posture, hand signals, and treat delivery, introduce towels or equipment gradually, and decide when to stop if the dog’s fear escalates.
Planning entry route and door controlSide-on posture and averted gazeReading response to first approachTreat offering and consent testingIntroducing towel and equipmentCriteria for aborting initial contactLesson 7Step-by-step jugular blood draw protocol: positioning on table or floor, head control methods, assistant roles, needle angle and depth, handling rebound movementPerform a jugular blood draw with safe positioning on table or floor, secure head and body control, and correct needle angle and depth. Coordinate with assistants to manage rebound movement and protect the airway.
Choosing table versus floor positioningHead and neck alignment and supportAssistant roles at forelimbs and bodyNeedle angle, depth, and stabilizationManaging coughing or sudden movementPost-draw pressure and bandage useLesson 8Pre-approach assessment and patient reading: evaluating body language, stress score, and medical history reviewLearn how to assess a nervous dog before contact by reading posture, facial tension, and vocalizations, combining this with stress scoring and medical history to predict handling risk and choose the safest restraint plan.
Baseline observation from a distanceKey canine fear and anxiety signalsUsing stress scores to guide handlingReviewing medical and behavior historyPredicting handling and restraint riskLesson 9Step-by-step cephalic blood draw protocol: positioning, vein visualization, tourniquet use, assistant positioning, needle insertion and withdrawal, minimizing movementFollow a precise cephalic blood draw sequence, from positioning and vein visualization to tourniquet use, needle placement, and withdrawal, while coordinating with an assistant to minimize movement and prevent complications.
Choosing side and limb for venipunctureHandler and assistant body positioningVein distension and tourniquet techniqueNeedle angle, insertion, and stabilizationTube filling, release, and needle withdrawalManaging hematoma or failed attemptsLesson 10Personnel roles and communication: assigning handler, recorder, veterinarian; clear commands and escape-route awarenessDefine clear roles for each team member, including primary handler, venipuncturist, and recorder. Practice concise verbal cues, confirm the plan, and maintain awareness of exits and backup options if the dog becomes unsafe.
Assigning handler and venipuncturistRecorder and timekeeper responsibilitiesPre-procedure briefing and role rehearsalStandard verbal cues and confirmationsMaintaining line-of-sight and exit accessLesson 11Clinic environment setup: room layout, lighting, non-slip flooring, calming pheromones, equipment checklist (muzzles, towels, gloves, cat/bandage tape, syringe setup)Set up a calm, efficient clinic space with safe room layout, stable surfaces, and appropriate lighting. Prepare non-slip footing, pheromone support, and a complete restraint and venipuncture equipment kit before bringing the dog in.
Room layout and escape-route controlLighting, noise, and visual distractionsNon-slip mats and table surface choicesPheromone diffusers and sound maskingPre-loading venipuncture and restraint kit
