Transgene Course
Gain expertise in creating transgene-based tomato lines resistant to fungal diseases. The course covers construct design, Agrobacterium-mediated transformation, molecular screening techniques like PCR and qPCR, disease phenotyping assays, and critical biosafety plus regulatory requirements to develop compliant, effective resistant varieties for biological research and applications.

4 to 360 hours flexible workload
valid certificate in your country
What will I learn?
This course provides a practical guide to designing, transforming, and validating tomato lines with lasting resistance to fungal diseases. Key topics include transgene construct design, promoter selection, RNAi and HIGS methods, transformation protocols, regeneration techniques, molecular validation, phenotyping, and essential biosafety, risk assessment, plus regulatory compliance for successful outcomes.
Elevify advantages
Develop skills
- Design optimised transgene constructs with suitable promoters, markers, and targeting signals.
- Perform tomato transformation using Agrobacterium methods and efficient regeneration processes.
- Validate transgene activity through PCR, qPCR, RT-qPCR, Western blots, ELISA, and reporter assays.
- Conduct disease resistance tests with proper inoculation, phenotyping, and statistical analysis.
- Implement biosafety protocols, risk evaluations, and regulatory documentation preparation.
Suggested summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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