Restriction Enzymes Course
Gain confidence in using restriction enzymes for cloning in biological sciences. This course covers designing primers with restriction sites, selecting suitable vectors, planning accurate digests, optimising ligations and transformations, plus troubleshooting and verifying constructs through colony screening, PCR, and sequencing for reliable lab results.

4 to 360 hours flexible workload
valid certificate in your country
What will I learn?
This course provides a streamlined, hands-on guide from primer design to confirmed constructs using restriction enzymes. You will learn to create primers ready for restriction, select expression vectors, execute precise digests, refine ligations, perform colony PCR and minipreps, conduct diagnostic checks, troubleshoot issues, and consider other cloning techniques for consistent outcomes in your lab work.
Elevify advantages
Develop skills
- Design restriction cloning with precise enzyme sites for clean directional inserts.
- Master PCR primers that are Tm-balanced, frame-safe, and include restriction sites.
- Select optimal plasmids, tags, and promoters for efficient expression.
- Perform efficient digests, ligations, and E. coli transformations.
- Verify clones using digests, colony PCR, and sequencing.
Suggested summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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