Transgene Course
Gain expertise in creating transgene-based tomato lines resistant to fungal diseases. The course covers transgene construct design, plant transformation protocols, molecular screening methods, disease resistance testing, biosafety protocols, and regulatory requirements to develop safe and effective resistant varieties for biological research and applications. This hands-on training equips you with skills for real-world biotech projects.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
This course provides a practical guide to designing, transforming, and validating tomato plants with strong resistance to fungal diseases. You will master transgene construct design, promoter selection, RNAi and HIGS methods, plant transformation processes, molecular validation techniques, disease phenotyping, and essential biosafety, risk evaluation, plus regulatory compliance for successful outcomes.
Elevify advantages
Develop skills
- Design effective transgene constructs with optimal promoters, markers, and targeting.
- Perform tomato transformation using Agrobacterium and efficient regeneration techniques.
- Validate gene expression through PCR, qPCR, RT-qPCR, Western blot, ELISA, and reporters.
- Conduct disease resistance assays with proper inoculation, phenotyping, and data analysis.
- Implement biosafety measures, risk assessments, and regulatory documentation standards.
Suggested summary
Before starting, you can change the chapters and workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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