PCR Techniques Course
Gain expertise in PCR and qPCR techniques, covering everything from sample handling and nucleic acid extraction to assay design, contamination prevention, optimised thermal cycling, plate layouts, data analysis, and quality documentation for producing reliable, verifiable results in busy diagnostic labs.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
This course delivers hands-on training in PCR and qPCR, including assay design, nucleic acid extraction methods, contamination prevention strategies, thermal cycler setup, protocol validation, plate layout planning, control usage and interpretation, troubleshooting of poor results, and robust quality management practices to ensure trustworthy data in high-volume settings.
Elevify advantages
Develop skills
- Design effective PCR/qPCR assays by selecting targets, primers, and probes quickly.
- Execute contamination-free nucleic acid extractions while preserving RNA quality.
- Optimise qPCR setups including master mixes, thermal cycling, and plate designs.
- Analyse PCR/qPCR results accurately with Ct values, controls, and quality checks.
- Implement laboratory quality controls like SOPs, records, and biosafety protocols.
Suggested summary
Before starting, you can change the chapters and workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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