Transgene Course
This course provides a comprehensive guide to developing durable fungal-resistant tomato lines through transgene technology. Participants master construct design with optimal promoters and RNAi strategies, Agrobacterium-mediated transformation and regeneration, molecular validation via PCR and protein assays, disease phenotyping protocols, and essential biosafety risk assessments with regulatory dossier preparation for compliant, effective applications in biological research.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
Gain practical skills in designing transgene constructs, transforming tomatoes, validating expression, phenotyping resistance, and ensuring biosafety compliance for fungal-resistant tomato lines using promoters, RNAi, HIGS, Agrobacterium methods, PCR screening, and regulatory documentation.
Elevify advantages
Develop skills
- Design optimised transgene constructs with promoters, markers, and targeting.
- Perform tomato transformation using Agrobacterium and regeneration techniques.
- Validate transgenes with PCR, qPCR, Western blots, and ELISA assays.
- Conduct disease resistance assays with inoculation and phenotyping analysis.
- Implement biosafety protocols and prepare regulatory documentation.
Suggested summary
Before starting, you can change the chapters and workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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