Restriction Enzymes Course
This course equips you with essential skills in restriction enzyme cloning for biological projects. You will learn to design primers and vectors, execute digests and ligations, transform cells, screen colonies, and troubleshoot issues to produce verified expression constructs reliably in your lab work.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
Gain practical expertise in restriction enzyme techniques, from designing primers with enzyme sites and selecting vectors, to performing digests, ligations, transformations, colony PCR, minipreps, and diagnostic checks, while mastering troubleshooting and alternative cloning for consistent lab success.
Elevify advantages
Develop skills
- Design restriction cloning with precise enzyme sites for clean directional inserts.
- Master PCR primers that are Tm-balanced, frame-safe, and include restriction sites.
- Select optimal plasmids, tags, and promoters for efficient expression.
- Perform efficient digests, ligations, and E. coli transformations.
- Verify clones using digests, colony PCR, and sequencing.
Suggested summary
Before starting, you can change the chapters and workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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