Electrophoresis Course
Gain expertise in electrophoresis techniques essential for molecular biology labs. This course covers agarose gels, SDS-PAGE, western blotting, PCR and RFLP design, troubleshooting, data analysis, and documentation to produce reliable, reproducible, and publication-quality results from DNA and protein samples.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
This course equips you with hands-on skills to perform agarose gel electrophoresis, SDS-PAGE, and western blotting confidently. You will explore fundamental principles, gel and buffer choices, PCR product preparation, RFLP methods, optimisation techniques, troubleshooting common problems, quantitative analysis of proteins, data interpretation, and proper documentation for clear, repeatable, and professional-grade outcomes in molecular biology.
Elevify advantages
Develop skills
- Plan agarose and SDS-PAGE gels by selecting percentages, buffers, ladders, and voltages efficiently.
- Design PCR and RFLP experiments with suitable primers, amplicon lengths, and markers for distinct bands.
- Perform reliable western blots through optimised transfer, blocking, antibody use, and detection methods.
- Quickly troubleshoot issues in gels and blots like smearing, faint bands, or excessive background.
- Document experiments properly by naming files, storing gels and blots, and recording metadata.
Suggested summary
Before starting, you can change the chapters and workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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