Transgene Course
Gain expertise in transgene technology for tomato fungal resistance. The course covers construct architecture, Agrobacterium transformation, molecular validation via PCR and protein assays, disease phenotyping, biosafety protocols, and regulatory documentation to develop durable, compliant resistant plant lines for biological research and applications.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
This course offers a practical guide to designing, transforming, and validating tomato lines resistant to fungal diseases. Key topics include construct design, promoter selection, RNAi and HIGS methods, transformation processes, regeneration techniques, molecular screening, phenotyping, and biosafety plus regulatory compliance for successful outcomes.
Elevify advantages
Develop skills
- Design effective transgene constructs with optimal promoters, markers, and targeting.
- Perform tomato transformation using Agrobacterium and efficient regeneration methods.
- Validate transgenes through PCR, qPCR, RT-qPCR, Western blot, ELISA, and reporters.
- Conduct disease resistance assays with proper inoculation, phenotyping, and data analysis.
- Implement biosafety measures and prepare regulatory risk assessments and dossiers.
Suggested summary
Before starting, you can change the chapters and workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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