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Restriction Enzymes Course

Restriction Enzymes Course
from 4 to 360h flexible workload
valid certificate in your country

What will I learn?

Gain practical skills in restriction enzyme cloning: design primers with sites, select expression vectors, plan digests and ligations, master colony PCR and minipreps, run diagnostic checks, troubleshoot problems, and learn alternative methods for consistent lab success.

Elevify advantages

Develop skills

  • Design restriction cloning with precise enzyme sites for clean directional inserts.
  • Master PCR primers that are Tm-balanced, frame-safe, and include restriction sites.
  • Select optimal plasmids, tags, and promoters for quick expression.
  • Perform efficient digests, ligations, and E. coli transformations.
  • Verify clones using digests, colony PCR, and sequencing.

Suggested summary

Before starting, you can change the chapters and workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.
Workload: between 4 and 360 hours

What our students say

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Very great course. Lots of rich information.
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