DNA Structure Course
Gain mastery over DNA structure, starting from the double helix and base pairing, through melting temperature, GC content, and reverse complements. Discover how replication fidelity, mismatches, and primer design affect PCR, cloning, and sequencing workflows—perfect for progressing in molecular biology and related fields.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
This course provides a quick, hands-on understanding of DNA double helix basics, base pairing rules, and thermodynamics, applying them to everyday lab tasks. You will learn to analyze sequences, compute GC content and melting temperature, create reverse complements, spot structural issues, and grasp replication accuracy. By the end, you can confidently design primers, fix PCR problems, boost sequencing results, and prepare for cloning projects.
Elevify advantages
Develop skills
- DNA sequence analysis: rapidly calculate GC content, Tm, and identify problematic motifs.
- Complement design: produce precise complementary and reverse-complement sequences efficiently.
- PCR optimization: create reliable primers while preventing dimers and hairpins.
- Replication fidelity: analyze mismatches, proofreading, and mismatch repair effects.
- Lab cloning skills: use base pairing for sticky-end, blunt-end, and adapter ligations.
Suggested summary
Before starting, you can change the chapters and workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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