Transgene Course
Gain expertise in creating transgene constructs for tomato fungal resistance. The course covers construct design, Agrobacterium transformation, molecular validation techniques like PCR and qPCR, disease phenotyping assays, and essential biosafety plus regulatory compliance to develop durable, safe resistant tomato varieties for biological research and applications.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
This course offers a practical guide to designing, transforming, and validating tomato lines with lasting resistance to fungal diseases. You'll master construct design, promoter selection, RNAi and HIGS methods, transformation processes, regeneration techniques, molecular screening, phenotyping, plus biosafety, risk evaluation, and regulatory paperwork for compliant outcomes.
Elevify advantages
Develop skills
- Design effective transgene constructs with optimal promoters, markers, and targeting.
- Perform tomato transformation using Agrobacterium and efficient regeneration methods.
- Validate transgenes via PCR, qPCR, RT-qPCR, Western blot, ELISA, and reporter assays.
- Conduct disease resistance tests with proper inoculation, phenotyping, and data analysis.
- Ensure compliance through biosafety protocols, risk assessments, and regulatory dossiers.
Suggested summary
Before starting, you can change the chapters and workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
As suas aulas são perfeitas. Adquiri o pacote de um ano e, finalmente, tenho a oportunidade de acompanhar diversos temas do meu interesse sem precisar mudar de plataforma... agradeço por tudo o que fazem, já recomendei vocês a outras pessoas...

Giulio CarloAluno de Marketing Digital
Eu gosto de como as lições são direto ao ponto e como eu consigo alterar capítulos e pular conteúdos que não preciso.

Mariana FerresAluna de Fotografia
Gosto do conteúdo e do modo de apresentação e transcrição de vídeos, o que acelera o processo!

Luciana AlvarengaAluna de Design de Unhas
A plataforma é rápida, simples de usar. A diversidade de conteúdo e os vídeos complementares ajudam muito no aprendizado.

Giulio CarloAluno de Engenharia de Prompt
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