Transgene Course
Gain expertise in creating transgene constructs for tomato fungal resistance. The course covers construct design, Agrobacterium transformation, molecular validation techniques like PCR and qPCR, disease phenotyping assays, and essential biosafety plus regulatory compliance to develop durable, safe resistant tomato varieties for biological research and applications.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
This course offers a practical guide to designing, transforming, and validating tomato lines with lasting resistance to fungal diseases. You'll master construct design, promoter selection, RNAi and HIGS methods, transformation processes, regeneration techniques, molecular screening, phenotyping, plus biosafety, risk evaluation, and regulatory paperwork for compliant outcomes.
Elevify advantages
Develop skills
- Design effective transgene constructs with optimal promoters, markers, and targeting.
- Perform tomato transformation using Agrobacterium and efficient regeneration methods.
- Validate transgenes via PCR, qPCR, RT-qPCR, Western blot, ELISA, and reporter assays.
- Conduct disease resistance tests with proper inoculation, phenotyping, and data analysis.
- Ensure compliance through biosafety protocols, risk assessments, and regulatory dossiers.
Suggested summary
Before starting, you can change the chapters and workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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