Electrophoresis Course
Gain expertise in electrophoresis techniques essential for molecular biology labs. This course covers agarose gels, SDS-PAGE, western blotting, PCR and RFLP design, troubleshooting, data analysis, and documentation to produce reliable, reproducible, and publication-quality DNA and protein results from basic principles to advanced applications.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
This course equips you with hands-on skills to set up and execute agarose gels, SDS-PAGE, and western blots effectively. You will explore electrophoresis fundamentals, gel and buffer choices, PCR product preparation, RFLP methods, optimisation techniques, troubleshooting common problems, quantitative protein analysis, data interpretation, and proper documentation for consistent, high-quality molecular biology outcomes.
Elevify advantages
Develop skills
- Plan agarose and SDS-PAGE gels by selecting percentages, buffers, ladders, and voltages.
- Design PCR and RFLP assays with suitable primers, amplicon sizes, and markers for clear results.
- Perform high-quality western blots through optimised transfer, blocking, antibodies, and detection.
- Troubleshoot gel and blot issues like smearing, faint bands, or high backgrounds swiftly.
- Document experiments properly by naming files, storing gels and blots, and recording metadata.
Suggested summary
Before starting, you can change the chapters and workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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