Restriction Enzymes Course
Master restriction enzymes fi confident cloning inna yuh biology projects. Design primers, pick vectors, plan digests, an fix up ligation, transformation, an colony screening fi build an verify expression constructs wid ease an reliability.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
Dis course gi yuh a quick, hands-on route from primer design to solid constructs. Yuh guh learn how fi design primers ready fi restriction, pick expression vectors, plan sharp digests, an optimize ligations. Master colony PCR, minipreps, an diagnostic digests, den troubleshoot problems an check out other cloning ways fi reliable results inna yuh lab work.
Elevify advantages
Develop skills
- Restriction cloning design: plan precise enzyme sites fi clean, directional inserts.
- PCR primer mastery: build Tm-balanced, frame-safe primers wid restriction sites.
- Vector strategy: choose top plasmids, tags, an promoters fi quick expression.
- Ligation an transformation: set up efficient digests, ligations, an E. coli uptake.
- Clone verification: use digests, colony PCR, an sequencing fi confirm constructs.
Suggested summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students are saying
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