Lesson 1Calibration, linearity, an limit-ah-detection concepts fi analyzersIntroduces calibration concepts, linearity verification, an limits ah detection an quantitation fi analyzers, highlightin protocols, acceptance criteria, an how dese parameters support method validation an ongoin quality assurance, yuh know.
Primary an secondary calibratorsCalibration curves an verificationLinearity assessment an reportable rangeLimit ah detection an quantitationOngoing monitrin ah analytical performanceLesson 2Data interfaces: LIS integration, middleware rules, an result routinExplores LIS connectivity, middleware configuration, an secure data routin, focusin on interface validation, rule-based result handlin, error flags, an maintainin data integrity, traceability, an cybersecurity in clinical workflows, fi security.
Basic LIS–analyzer communication modelsMiddleware rules fi result handlinResult routin, auto-filin, an holdsInterface validation an change controlData security, backups, an audit trailsLesson 3Clinical chemistry analyzers: BMP assays, ion-selective electrode fi potassiumReviews clinical chemistry analyzer operation fi BMP assays, focusin on photometric an enzymatic methods, ion-selective electrode measurement ah potassium, common interferences, calibration, an internal quality checks fi reliable results, no doubt.
Core components ah chemistry analyzersPhotometric an enzymatic BMP assaysIon-selective electrode theory fi K+Reagent integrity an carryover issuesInternal QC an analyzer performance checksLesson 4Maintenance routines, preventive maintenance logs, an troubleshootinCovers daily, weekly, an periodic maintenance tasks, documentation ah preventive maintenance logs, an structured troubleshootin strategies to minimize downtime, extend analyzer lifespan, an preserve accuracy an regulatory compliance, keepin it runnin.
Daily an weekly maintenance checklistsDocumentin preventive maintenance logsTroubleshootin systematic vs random errorsEscalation to vendor technical supportRegulatory an accreditation documentationLesson 5Delta checks, repeat testin rules, an reflex testin algorithmsExplains delta checks, repeat testin criteria, an reflex testin algorithms, emphasizin evidence-based thresholds, clinical context, an how dese tools improve error detection, diagnostic yield, an efficient test utilization, yuh see.
Principles an setup ah delta checksDefinin repeat testin thresholdsDesignin reflex testin algorithmsBalancin sensitivity an workloadDocumentin rules an clinician communicationLesson 6Principles ah hematology analyzers: CBC methodology an common artifactsDescribes hematology analyzer principles fi CBC, includin impedance an optical methods, sample flow, an common artifacts such as platelet clumps, cold agglutinins, an lipemic interference, plus strategies fi detection an correction, fi accuracy.
Impedance an optical countin principlesSample preparation an flow cell designRecognizin platelet clumps an clotsCold agglutinins an RBC agglutinationFlags promptin smear review an repeatsLesson 7Common pre-analytical interferences: hemolysis, lipemia, icterus an deir laboratory detectionAddresses pre-analytical interferences from hemolysis, lipemia, an icterus, detailin deir biochemical impact, detection by indices or visual inspection, rejection criteria, an strategies to minimize occurrence an misinterpretation, keepin results clean.
Mechanisms ah hemolysis in specimensLipemia effects on photometric assaysIcterus an bilirubin-related biasHIL indices versus visual inspectionSpecimen rejection an recollection rulesLesson 8Coagulation analyzers: PT/INR methodology (photometric vs mechanical clot detection)Compares coagulation analyzer technologies fi PT/INR, includin photometric an mechanical clot detection, reagent sensitivity, calibration to international standards, an factors weh cause spurious prolongation or instrument error flags, yuh know.
Coagulation cascade an PT/INR basicsPhotometric clot detection principlesMechanical clot detection systemsISI, INR calculation, an calibrationInterferences an problematic specimensLesson 9Analyte-specific problem: spurious hyperkalemia causes an investigation stepsFocuses on spurious hyperkalemia, outlinin pre-analytical, analytical, an post-analytical causes, stepwise investigation, confirmatory testin, an communication wid clinicians to avoid misdiagnosis an inappropriate treatment, fi patient safety.
Pre-analytical causes ah false hyperkalemiaAnalytical an instrument-related issuesComparin serum, plasma, an whole bloodAlgorithm fi investigatin high potassiumReportin, comments, an clinician follow-upLesson 10Understandin index flags from analyzers an autoverification rulesExplains analyzer index flags, includin hemolysis, lipemia, icterus, an instrument error codes, an how autoverification rules use dese flags, delta checks, an critical limits to safely release or hold results fi review, keepin it reliable.
Types ah analyzer index an system flagsFlag thresholds an analytical limitationsDesignin safe autoverification rulesIncorporatin delta an critical limitsManual review an override procedures
