Transgene Course
Gain expertise in creating transgene-based tomato lines resistant to fungal diseases. The course covers transgene construct design, Agrobacterium-mediated transformation, molecular validation techniques like PCR and qPCR, disease phenotyping assays, biosafety protocols, and regulatory documentation to develop safe, effective resistant varieties for biological research and applications.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
This course offers a practical guide to designing, transforming, and validating tomato lines with strong resistance to fungal diseases. Key topics include transgene construct design, promoter selection, RNAi and HIGS methods, transformation protocols, regeneration techniques, molecular validation, phenotyping, biosafety measures, risk evaluation, and regulatory compliance for successful outcomes.
Elevify advantages
Develop skills
- Design optimised transgene constructs with suitable promoters, markers, and targeting signals.
- Perform tomato transformation using Agrobacterium methods and efficient regeneration processes.
- Validate transgene activity through PCR, qPCR, RT-qPCR, Western blot, ELISA, and reporter assays.
- Conduct disease resistance tests with proper inoculation, phenotyping, and statistical analysis.
- Implement biosafety protocols, risk assessments, and prepare regulatory dossiers for compliance.
Suggested summary
Before starting, you can change the chapters and workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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