Restriction Enzymes Course
This course teaches you to master restriction enzymes for reliable cloning in biology projects. You will design primers with restriction sites, select suitable vectors, execute precise digests and ligations, handle transformations, and verify constructs through colony PCR, minipreps, and diagnostic tests. Gain skills to troubleshoot issues and achieve reproducible results in your lab work.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
Gain practical skills in restriction enzyme cloning: design primers ready for restriction sites, select expression vectors, plan accurate digests, optimize ligations, perform colony PCR and minipreps, run diagnostic digests, troubleshoot problems, and learn alternative cloning techniques for dependable results in your biology lab projects.
Elevify advantages
Develop skills
- Design restriction cloning with precise enzyme sites for clean directional inserts.
- Master PCR primers that are Tm-balanced, frame-safe, and include restriction sites.
- Select optimal plasmids, tags, and promoters for quick protein expression.
- Perform efficient digests, ligations, and E. coli transformations.
- Verify clones using digests, colony PCR, and sequencing confirmation.
Suggested summary
Before starting, you can change the chapters and workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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