PCR Techniques Course
4.5/5
This course equips you with essential PCR and qPCR skills, from sample handling and extraction to assay setup, data analysis, and quality control. Master designing assays, preventing contamination, optimizing runs, interpreting results, and maintaining lab standards for reliable, high-throughput testing in professional settings.

4.5 in the evaluation
from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
Gain hands-on training in PCR and qPCR methods, covering assay design, nucleic acid extraction, contamination prevention, thermal cycling, protocol validation, plate layouts, control usage, troubleshooting, and quality documentation for trustworthy results.
Elevify advantages
Develop skills
- Design effective PCR/qPCR assays by selecting optimal targets, primers, and probes.
- Execute contamination-free nucleic acid extractions while preserving sample integrity.
- Configure and refine qPCR experiments including master mixes, cycling conditions, and layouts.
- Analyze PCR/qPCR outputs with accurate Ct values, controls, and quality checks.
- Implement robust lab practices like SOPs, records, biosafety, and result reporting.
Suggested summary
Before starting, you can change the chapters and workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
Your lessons are perfect. I purchased the one-year package and finally have the opportunity to follow various topics of my interest without needing to change platforms... I thank you for everything you do, I've already recommended you to other people...

Giulio CarloDigital Marketing Student
I like how the lessons are straight to the point and how I can change chapters and skip content I don't need.

Mariana FerresPhotography Student
I like the content and the way videos are presented and transcribed, which speeds up the process!

Luciana AlvarengaNail Design Student
The platform is fast, simple to use. The diversity of content and complementary videos really help with learning.

André FelipePrompt Engineering Student
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