Lesson 1Automated chemistry analyzers: photometric and ion-selective electrode methods for Na, K, Cl, creatinine, glucoseCovers main parts of auto chemistry machines, focusing on light measure and ion pick electrode methods for sodium, potassium, chloride, creatinine, and glucose, including setup, blocks, and basic fix in Gambian labs.
Work flow of a usual chemistry machine runLight measure tests for creatinine and glucoseIon pick electrodes for Na, K, and ClUsual blocks and signs in chemistry testsSetup and check for light measure and ISELesson 2Running a small batch: batching strategy, sample order, and throughput considerationsDescribes how to plan and run small testing batches, including grouping tests, setting sample order, handling controls and setups, and balancing speed with wait time and reagent save in Gambia.
Defining a batch and grouping like testsPlacing setups, controls, and patientsBest sample order for urgent samplesBalancing speed with wait timeCutting reagent waste in small batchesLesson 3Automated hematology analyzers: principles (impedance, flow cytometry), CBC parameters measuredIntroduces auto blood count machines, describing block and flow light principles, how CBC parts are made, and key signs, limits, and auto-check rules that guide slide look and hand check in Gambia.
Block counting for red cells and plateletsFlow light and scatter for white cellsMeasured vs worked out CBC partsUsual blood count signs and error notesAuto slide rules and hand check needsLesson 4Calibration, reagent management, and analyzer startup/shutdown proceduresExplains usual setup, reagent prep, loading, and stock control, along with standard machine start and stop steps that guard tool work, cut down time off, and make sure true patient results in Gambian labs.
Main vs extra setup and how oftenReagent storage, batch changes, and recordsSetting and checking setup curvesDaily start checks and back measuresStop, clean cycles, and power off stepsLesson 5Troponin I testing methods: immunoassays and timing considerationsCovers troponin I immune test basics, including high-sense methods, sample handling, timing of repeat measures, and reading rising or falling patterns in suspected heart attack cases in Gambia.
Troponin I body role and clinic meaningUsual vs high-sense troponin testsSample type, steady, and hemolysis effectsTiming repeat troponin in heart casesReading change amounts and decision limitsLesson 6HbA1c measurement methods: HPLC, immunoassay, enzymatic methods, and specimen requirementsDetails HbA1c measure by HPLC, immune test, and enzyme methods, comparing basics, good points, and limits, and reviews sample needs, steady, and factors that can wrongly change HbA1c values in Gambia.
Basics of charge-swap HPLC for HbA1cImmune test HbA1c measure basicsEnzyme HbA1c methods and work flowSample type, storage, and steady for HbA1cBlood type changes and conditions affecting HbA1cLesson 7CK-MB assay principles and when to run with troponinExplores CK-MB test basics, clinic signs, and timing, and explains when CK-MB should be done with heart troponin to clear heart damage, rule out false highs, and help true reading in Gambian clinics.
CK-MB type body role and clinic meaningTest formats for CK-MB immune testsSample type, handling, and blocks for CK-MBTiming CK-MB vs troponin in chest pain checksReading clashing CK-MB and troponin resultsLesson 8Pipetting technique, maintenance of micropipettes, and minimizing carryoverFocuses on right pipetting skill, including angle, speed, and dip depth, along with usual upkeep, setup of small pipettes, and ways to cut carry-over and cross-dirt between samples in Gambia.
Forward and back pipetting best waysSetting volume, angle, and dip depthUsual clean and seal checkPipette setup checks and recordsStopping carry-over and mist formLesson 9CRP measurement techniques: immunoturbidimetric and high-sensitivity CRP basicsReviews CRP measure using immune-cloud methods and high-sense CRP tests, pointing out test basics, setup, clinic uses, and pre-test factors that affect result truth and reading in Gambian labs.
Immune-cloud CRP test basicHigh-sense CRP: method and usesSample type, steady, and handling for CRPTest measure range and dilute wayReading CRP vs hs-CRP clinic cutoffsLesson 10Using internal quality control materials: levels, acceptance ranges, Levey-Jennings charts and Westgard rulesExplains how to pick inside quality control levels, set okay ranges, and use Levey-Jennings charts with Westgard rules to spot steady and chance errors before sending patient results in Gambia.
Picking QC materials and amount levelsSetting target means and SD rangesPlotting and checking Levey-Jennings chartsUsing key Westgard rules dailyRecording QC fails and fix actions
