Transgene Course
Gain expertise in transgene technology for tomato fungal resistance. The course covers construct design, Agrobacterium transformation, regeneration, validation via PCR and phenotyping, disease assays, and essential biosafety plus regulatory compliance to develop durable, safe resistant varieties for biological research and applications.

flexible workload of 4 to 360h
valid certificate in your country
What will I learn?
This course provides a practical guide to designing, transforming, and validating tomato lines with lasting resistance to fungal diseases. You'll master construct design, promoter selection, RNAi and HIGS methods, transformation processes, molecular screening, phenotyping, plus biosafety, risk evaluation, and regulatory paperwork for compliant outcomes.
Elevify advantages
Develop skills
- Design effective transgene constructs with optimal promoters, markers, and targeting.
- Perform tomato transformation using Agrobacterium and efficient regeneration techniques.
- Validate transgenes through PCR, qPCR, RT-qPCR, Western blot, ELISA, and reporter assays.
- Conduct disease resistance tests with proper inoculation, phenotyping, and data evaluation.
- Implement biosafety protocols, risk assessments, and regulatory documentation standards.
Suggested summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workloadWhat our students say
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