Restriction Enzymes Course
Gain confidence in using restriction enzymes for cloning in biological sciences. Master primer design with restriction sites, vector selection, precise digests, ligation optimisation, transformation, and verification methods like colony PCR, minipreps, and sequencing to build and confirm expression constructs reliably in your projects.

flexible workload of 4 to 360h
valid certificate in your country
What will I learn?
This course offers a quick, hands-on guide from designing primers to confirming constructs using restriction enzymes. You'll learn to create primers ready for restriction, select suitable expression vectors, execute accurate digests, and perfect ligation techniques. Gain expertise in colony PCR, minipreps, diagnostic digests, troubleshooting issues, and alternative cloning strategies for dependable results in your lab work.
Elevify advantages
Develop skills
- Design restriction cloning with exact enzyme sites for directional inserts.
- Master PCR primers that are Tm-balanced, frame-safe, and include restriction sites.
- Select best plasmids, tags, and promoters for efficient expression.
- Perform effective digests, ligations, and E. coli transformations.
- Verify clones using digests, colony PCR, and sequencing.
Suggested summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workloadWhat our students say
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