High-Performance Liquid Chromatography (HPLC) Course
Gain mastery in HPLC for multi-component pharmaceuticals. Master column and mobile phase selection, gradient optimisation, detection techniques, troubleshooting, and validation to produce reliable, regulatory-compliant methods in a demanding lab setting. This focused course equips you with practical expertise for real-world applications.

4 to 360 hours flexible workload
valid certificate in your country
What will I learn?
This High-Performance Liquid Chromatography (HPLC) Course imparts hands-on skills for developing and refining methods for multi-component pharmaceuticals. You will cover column and mobile phase choices, gradient planning, sample prep, calibration with internal standards, validation including precision, accuracy, LOD/LOQ, troubleshooting, robustness testing, and preparing regulatory documentation in an intensive format.
Elevify advantages
Develop skills
- Develop robust HPLC methods for multi-component tablets.
- Optimise mobile phases with pH, buffers, and gradients for sharp peaks.
- Select and optimise detectors including UV, DAD, ELSD, or MS.
- Validate methods for accuracy, precision, LOD, and LOQ per guidelines.
- Troubleshoot issues like tailing, drift, and pressure problems swiftly.
Suggested summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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