Restriction Enzymes Course
Gain expertise in restriction enzymes to achieve reliable cloning results. The course covers designing effective primers, selecting vectors, planning digests, optimizing ligations and transformations, and verifying clones through colony screening, PCR, and sequencing. Ideal for biological sciences projects requiring precise construct development and troubleshooting skills.

4 to 360h flexible workload
certificate valid in your country
What will I learn?
This course provides a streamlined, hands-on guide from primer design to confirmed constructs using restriction enzymes. Participants will learn to create primers suited for restriction digestion, select appropriate expression vectors, execute accurate restriction digests, and refine ligation techniques. Additionally, master colony PCR, miniprep procedures, and diagnostic digests while addressing common issues and discovering backup cloning strategies for dependable, repeatable outcomes in laboratory settings.
Elevify advantages
Develop skills
- Design restriction cloning strategies with precise enzyme sites for directional inserts.
- Master PCR primer design featuring balanced melting temperatures, correct reading frames, and restriction sites.
- Select optimal expression vectors including plasmids, affinity tags, and promoters.
- Perform efficient restriction digests, ligations, and bacterial transformations.
- Verify clones using diagnostic digests, colony PCR, and DNA sequencing.
Suggested summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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