Proteomics Course
This course provides a comprehensive guide to cell-line proteomics, covering sample preparation through to biological insights. Participants master LC-MS/MS setup, DDA and DIA methods, experimental design, quality control, bioinformatics tools, and statistical analysis to produce reliable protein profiles and validate pathways related to inflammation and disease models.

4 to 360h flexible workload
certificate valid in your country
What will I learn?
Gain practical expertise in designing and running LC-MS/MS proteomics experiments for cell lines. Master high-resolution mass spectrometry setup, DDA/DIA optimization, study planning, and quantitative methods. Develop skills in bioinformatics, statistics, functional analysis, and validation to produce trustworthy, publication-ready protein datasets.
Elevify advantages
Develop skills
- Design efficient LC-MS/MS proteomics workflows with optimal methods, quantification, and enrichment.
- Configure Orbitrap or timsTOF instruments for DDA and DIA to ensure high-quality data.
- Process raw mass spectrometry files using database searches, FDR control, and reliable quantification.
- Perform statistical analysis on proteomics data to identify differential proteins and pathways.
- Validate protein hits through PRM/SRM assays, Western blots, and troubleshooting techniques.
Suggested summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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